Cell Transfection Reagents

TransIT^® -Express Transfection Reagent

Developed for high-throughput transfections

This reagent is gentle enough to place complexes directly on cells at the time of seeding. Transfections can be carried out in serum-containing media and do not require media changes. This results in a time saving of hours to days without sacrificing results.

Optimal transfection of hard-to-transfect primary keratinocytes

Using this specifically designed reagent results in 20-30% transfection efficiency with green fluorescent protein keratinocytes.

TransIT^® -293 Transfection Reagent

Designed for performing highly efficient transfections in HEK293 cells

Allows 65-75% efficiency while leaving the cells healthy.

TransIT -HeLaMonster™ Transfection Reagent

Reagent specifically designed for difficult-to-transfect HeLa cells

TransIT^®-HeLaMONSTER™ Transfection Reagent is a novel two-component system optimized to provide superior transfection efficiency without sacrificing overall health of the cells. The reagents are simple to use and results can be easily reproduced. HeLa cells have been inherently difficult to transfect, yet have remained a prevalent cell line in cell biological research. The specificity of the TransIT^®-HeLaMONSTER™ reagent makes this product an important alternative to other broad spectrum transfection reagents. The significant attributes of TransIT^® -HeLaMONSTER™ - specificity, efficiency, and low toxicity - establish HeLaMONSTER™ as the reagent of choice for transfecting HeLa cells.

For high-efficient small interfering RNA transfection

Features:

• Delivers siRNA targeted against both transient transfected and stable genes in a variety of cell lines while not effecting untargeted expression of similar genes - significantly reduced cell damage
• Ideal for all siRNA-mediated gene silencing studies
• No media change or serum addition required

Product Description

The novel TransIT^® -TKO reagent enables highly efficient siRNA transfection with significantly reduced levels of cell damage as compared to cationic-liposome based transfection reagents. Transfections are most effective when carried out in complete growth media without a media change or serum addition. When complexed with even low levels of siRNA, TransIT^® -TKO reagent successfully knocks out target gene expression in a variety of cell lines. Transgene expression can be silenced either when the siRNA is transfected before, concurrently or after the transfection of the expression vector. These unique features make TransIT^® -TKO transfection reagent ideal for all siRNA-mediated gene-silencing studies. It is the only transfection reagent designed to efficiently deliver small interfering RNA to cells in vitro. TransIT^® -TKO reagent, combined with the correct siRNA sequence against the target mRNA, can silence endogenous, stable or transient gene expression.

High, reproducible protein yield in suspension CHO and 293 cells

Features

• Ideal for biotherapeutic protein production
• Achieve high protein yield in suspension CHO and 293 cells
• Compatible with multiple CHO media formulations
• Reproducible protein expression with minimal optimization

Features and Advantages

• Specifically developed to achieve the highest transfection efficiency in NIH 3T3 cells
• Based on the TransIT^®-LT (Low Toxicity) technology
• Achieve higher transfection efficiencies and save time by transfecting in the presence of serum and eliminating media changes
• Reagents are supplied with an optimized protocol, and quality control tested on a standard NIH 3T3 cell line

Product Description

TransIT^®-3T3 Transfection Kit was specifically developed to achieve the highest transfection efficiency in NIH 3T3 cells. This kit, which consists of TransIT^®-3T3 Reagent and a 3T3 Authority Reagent, results in 55-65% efficiency using GFP as a reporter, with minimal cellular toxicity. Transfections should be performed in serum-containing media, with no media change. MIR 2180 provides sufficient amounts of both reagents to perform up to 500 transfections in 35 mm dishes.

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Features and Advantages

• Specifically developed to achieve the highest transfection efficiency in CHO-K1 cells
  
• Based on the TransIT^®-LT (Low Toxicity) technology
  
• Achieve higher transfection efficiencies and save time by transfecting in the presence of serum and eliminating media changes
• Reagents are supplied with an optimized protocol, and quality control tested on a standard CHO-K1 cell line

Product Description

TransIT^®-CHO Transfection Kit was specifically developed to achieve the highest transfection efficiency in CHO-K1 cells. This kit, which consists of TransIT^®-CHO Reagent and a CHO Mojo Reagent, results in 50-60% efficiency using GFP as a reporter, with minimal cellular toxicity. Transfections should be performed in serum-containing media, with no media change. MIR 2170 provides sufficient amounts of both reagents to perform up to 500 transfections in 35 mm dishes.

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Features and Advantages

• Specifically developed to achieve the highest transfection efficiency in COS-7 and COS-1 cells
  
• Based on the TransIT®-LT (Low Toxicity) technology
  
• Achieve higher transfection efficiencies and save time by transfecting in the presence of serum and eliminating media changes
  
• Supplied with an optimized protocol, and quality control tested on a standard COS-7 cell line

Product Description

TransIT^®-COS Transfection Kit was specifically developed to achieve the highest transfection efficiency in COS-7 and COS-1 cells. This kit, which consists of TransIT^®-COS Reagent and a COS Boss Reagent, results in 90-95% efficiency using GFP as a reporter, with minimal cellular toxicity. Transfections should be performed in serum-containing media, with no media change. MIR 2190 provides sufficient amounts of both reagents to perform up to 500 transfections in 35 mm dishes.

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Features and Advantages

• Specifically for efficiently transfecting a wide range of oligonucleotides into cells
• TransIT^®-Oligo Transfection Reagent results in significantly reduced levels of cell damage compared to other Leading transfection reagents
  
• Achieve higher transfection efficiencies and save time by transfecting in the presence of serum and eliminating media changes
  
• Supplied with an optimized protocol, and a ready-to-use single vial.

Product Description

TransIT^®-Oligo Transfection Reagent was specifically developed for efficiently transfecting a wide range of oligonucleotides and oligoribonucleotides in cells. TransIT^®-Oligo provides this high efficiency delivery with the same ease-of-use and high reproducibility found in the rest of the TransIT^® line of products. In addition, TransIT^®-Oligo reagent transfections do not require media changes and can be carried out in serum-containing media.

Oligonucleotides tested: phosphodiester DNA, phosphothioate DNA (sDNA), phosphothioate RNA (sRNA), 2'OMe RNA, 2'OMe RNA/sDNA Chimerics, and Morpholino/DNA duplexes.

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Features and Advantages

• Highest transfection efficiencies in a wide range of neural cell types
  
• Significantly reduced levels of cell damage compared to liposomal based transfection reagents
  
• Achieve higher transfection efficiencies and save time by transfecting in the presence of serum and eliminating media changes
  
• Quality control tested on a standardized Neuro-2a cell line

Product Description

TransIT^®-Neural™ Transfection Reagent is a novel formulation specifically developed to achieve superior transfection efficiency in a wide range of neural cell types without sacrificing cell viability. This ready-to-use reagent results in up to 85% efficiency using pGFP as the reporter plasmid. Highest efficiencies are achieved when transfections are performed in the presence of serum-containing media, with no media change. MIR 2140 provides sufficient reagent to perform up to 500 transfections in 12-well plates.

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Features and Advantages

• The TransIT^® Prostate Kit consists of the TransIT^® Prostate Reagent and the Prostate Boost Reagent. This is the only kit developed to achieve the highest transfection efficiencies in prostate cells.
  
• Optimized to achieve greater than 50% transfection efficiencies using GFP as a reporter in common prostate cancer cell lines: PC-3, DU 145 and LNCaP-FGC.
  
• Observe significantly reduced levels of cell damage compared to liposomal based transfection reagents.
  
• Achieve higher transfection efficiencies and save time by transfecting in the presence of serum and eliminating media changes.
  
• Supplied with an optimized protocol and ready-to-use reagents to complex directly with plasmid DNA.
  
• Quality tested with a standard DU 145 cell line to ensure consistent results.
  

Product Description

The TransIT^®-Prostate Transfection Kit is specifically optimized to provide superior transfection efficiency in prostate cells without sacrificing cellular health. Generally, prostate cell types have been moderately difficult to transfect, yet have remained a prevalent cell line in the cancer research field.

The specificity of the TransIT^®-Prostate Transfection Kit makes this product a desirable alternative to broad spectrum transfection reagents. The kit provides all the attributes of the trusted TransIT^® Reagent line: high efficiency, low toxicity, simplicity of use, and reproducibility. Transfections with the TransIT^® Reagents do not require media changes and can be carried out in serum-containing media. In addition, the TransIT^®-Prostate Transfection Kit is quality control tested on ATCC DU-145 cells. These significant features establish the TransIT^®-Prostate Transfection Kit as the product of choice for transfecting prostate cells. MIR 2130 provides sufficient reagent to perform up to 500 transfections in 35 mm wells.

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Features and Advantages

• Ideal for screening applications: High efficiency knockdown in a 96-well plate format allows rapid, simultaneous screening of multiple siRNA conditions including siRNA concentration, sequence and cell type in one plate.
  
• Customized: Plates are available in four working concentrations of TransIT-TKO^® Reagent for optimization with a wide range of cell types. The Titration Plate is available for testing all four levels at one time in a preliminary screen.
  
• Convenient: Easy to use, serum compatible protocol; can be performed in a single 96-well plate or using a two step transfer method.
• Consistent performance: Efficient and reproducible siRNA delivery among multiple wells in a plate for performing replicate samples.
  

Product Description

The TransIT-TKO^® Reagent is supplied as a dry film on clear plastic, sterile, 96-well plates which have flat-bottom wells suitable for cell culture. Each plate is supplied individually vacuum sealed in a foil pouch to maintain sterility and ensure effectiveness. Researchers can test up to eight different siRNA sequences in triplicate format, at four different levels of reagent, from L1 (lowest) to L4 (highest), using the TransIT-TKO^® HTS-96 Titration Plate (MIR 2530), on their particular cell type. Once the optimal level has been determined based on the highest efficiency of transfection with the lowest cellular toxicity, additional plates can be purchased at the desired level. Transfections can be performed using a single TransIT-TKO^® HTS-96 Plate or using a two step transfer method, where the TransIT-TKO^® HTS-96 Plate is used as a reagent plate to form complexes and transfer to the plate of choice.

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Features and Advantages

• Developed specifically for high efficiency RNA delivery to mammalian cells.
  
• Low toxicity reagent proven to work effectively in a wide range of cell types.
  
• Achieve higher transfection efficiencies and save time by transfecting in the presence of serum and eliminating media changes.
  
• The reagents are supplied ready-to-use with an optimized protocol.
  

Product Description

The TransIT^®-mRNA Transfection Kit is optimized for efficient delivery of various types of RNA to mammalian cells. Both mRNA and viral RNA of various lengths can be delivered effectively to a wide range of cell types. In addition, transfection of mRNA leads to high level protein expression with minimal cytotoxicity. As an added benefit, this kit has been optimized for use in the presence of serum containing media thus eliminating media changes or serum addition. MIR 2250 provides sufficient reagents to perform up to 500 transfections per well of a 12-well plate.

Cell lines successfully transfected: A549, CHO-K1, COS-7, HEK 293, HeLa, HepG2, NIH 3T3, and Vero cell lines.


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Features and Advantages

• Developed specifically for high efficiency siRNA delivery to mammalian cells.
  
• Unique lipopolyplex formulation offers advantages in select cell types.
  
• Low toxicity reagent proven to work effectively in a wide range of cell types.
  
• Achieve higher transfection efficiencies and save time by transfecting in the presence
  of serum and eliminating media changes.
  
• The reagents are supplied with an optimized protocol, and a ready-to-use single vial.
  

Product Description

TransIT^®-siQUEST™ Transfection Reagent enables highly efficient siRNA transfection with significantly reduced levels of cell damage as compared to cationic-liposome based transfection reagents. Transfections are most effective when carried out in complete growth media, with no media change or serum addition required. TransIT^®-siQUEST™ Reagent efficiently delivers siRNA to a wide range of cell lines, promoting high levels of target gene expression knockdown. These unique features make the TransIT^®-siQUEST™ Transfection Reagent ideal for siRNA-mediated gene silencing studies. The TransIT^®-siQUEST™ Reagent is a complement to Mirus' popular TransIT-TKO^® siRNA Transfection Reagent. Each unique formulation provides a distinct transfection profile for high efficiency, broad-spectrum siRNA delivery.

Cell Lines Successfully Transfected: A549, CHO-K1, COS-7, HEK 293, HeLa, Hepa1c1c7, HepG2, MCF-7, NIH 3T3, RAW 264.7, and Vero cells.

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