products | nucleic acid and protein tools | Nucleic Acid Purification | AquaPlasmid™
 |
Nucleic Acid Purification
AquaPlasmid™
-
A novel aqueous solution-based technology for isolation and purification of ultrapure plasmid DNA
| AquaPlasmid™ Plasmid Prep Kits | ||
 |
Introduction | |
|
Comparison | |
|
Order Information | |
|
Downloads | |
|
FAQ | |
Overview
AquaPlasmid™ is a novel aqueous solution-based techology for isolation and purification of ultrapure plasmid DNA. It combines high quality results with easy handling and significant savings in terms of material costs, time and labor. The key features are:
- It's different
Isolation and purification based on selective extraction in aqueous solution rather than binding on solid support.
- It's simple
Cells are lysed, debris is precipitated, and ultrapure plasmid DNA is precipitated.
- It's easy
No columns, no resins, no slurries, no beads, no filters, no membranes, no needles, no cartridges, no manifolds, no HPLC.
- It's scalable
One single AquaPlasmid kit does all: mini, midi, maxi and 96-well preps.
- It's fast
Less than 25 minutes from pelleting the bacteria to ultrapure plasmid DNA.
- It saves resource
No phenol, no chloroform, no saturated butanol, no ethidium bromide, no cesium chloride, no guanidine hydrochloride, no buffer 1,2,3,4.
- It delivers ultrapure DNA
Excellent purity - suitable for all common applications in molecular biology
- It delivers high yield
Excellent quantities of 50 to 100 µg ultrapure plasmid DNA from 10 ml bacterial overnight culture.
- Its economic
AquaPlasmid™ kits are priced less than 1/3 compared with other plasmid DNA isolation kits - that's 50 ct per mini prep!
Facts and figures of AquaPlasmid™ in comparison with DNA purification kits from a leading competitor. |
||
Comparison |
Main competitor's kits |
AquaPlasmid™ kit |
Isolation Mechanism |
Selective Binding |
Selective Extraction |
Isolation Format |
Solid Support |
Aqueous Solution |
List Price per mini prep |
1.33 - 3.76 €* |
0.50 - 0.70 € |
RNase A to be added |
Yes |
No |
Guanidine HCl |
Yes |
No |
Scalable |
No |
Yes |
Number of Solutions in the kit |
6 - 7 |
2 |
Isolation Time (min) |
30 (mini) |
25 (mini) |
Yield (µg DNA / ml culture) |
2 - 4 (LB medium) |
2 - 5 (LB medium) |
Purity (A260 / A280) |
1.8 - 2.0 |
1.9 - 2.0 |
Recommended applications |
||
Sequencing |
Yes |
Yes |
PCR |
Yes |
Yes |
Cloning |
Yes |
Yes |
Restriction digestion |
Yes |
Yes |
Transformation |
Yes |
Yes |
Tranfection of most cells |
Partly* |
Yes |
Library construction |
Partly* |
Yes |
 |
order information |
| ORDER INFORMATION, SHIPPING & STORAGE: | |||
| order# | description | amount |
price |
| 1001MT | AquaPlasmid™ DNA Purification Kit Trial | 1 kit (e.g. 10 MINI Preps) |
15 € |
| 1010MT | AquaPlasmid™ DNA Purification Kit Small | 1 kit (e.g. 50 MINI Preps) |
35 € |
| 1030MT | AquaPlasmid™ DNA Purification Kit Large | 1 kit (e.g. 500 MINI Preps) |
249 € |
| shipped at RT; store at RT | |||
Downloads:
Material Safety Data Sheets (MSDS)
Handbook
- AquaPlasmid Handbook (pdf, 1,3 MB, last update 10.2006)
Info-Flyer
- AquaPlasmid Info-Flyer, (pdf, 217 kb)
| AquaPlasmid™ Plasmid Prep Kits | ||
|
Introduction | |
|
Comparison | |
|
Order Information | |
|
Downloads | |
|
FAQ | |
| << RNA Transcription | MobiSpin Columns for DNA Purification >> |
See also chapter 6 of our online catalog.
home | search | order | Impressum |
©2008 MoBiTec GmbH • All rights reserved • www.mobitec.com