products | nucleic acid and protein tools | Optimal Gel and Blot Staining
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Nucleic Acid and Protein Tools
Optimal Gel and Blot Staining
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Deep Blue - Colloidal Coomassie |
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Deep Silver Stain |
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Snow White Reversible Zinc Stain |
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Enlight Fluorographic Enhancer |
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NOWA Chemiluminescent Western |
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ProTect - DNA Protector |
Deep Blue - Colloidal Coomassie
Get the best Coomassie gels fast and easy!
Product description:
Deep Blue is the easiest way to Coomassie stain your protein gels. It´s sensitive, it doesn´t smell, it´s fast and it´s destain free! The equilibrium of the blue dye in Deep Blue is primarily driven towards its solid colloid state, leaving the solutuion almost colorless. Protein bands inside a gel accumulate these trace amounts of dye quickly and develop a dark blue stain while the rest of the gel remains clear. Watch your bands develop before your eyes, directly in the staining tray. After one hour the staining is complete and the gel can be dried directly. It is also possible to further increase the sensitivity of Deep Blue by an additional water wash step to detect even the weakest bands Deep Blue offers the best results for the best price! It can be reused for more than one gel!
Deep Blue is stable at room temperature for at least one year.
conventional Coomassie Blue + destain.
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Deep Silver Stain
High-sensitive gel staining with one single solution!
Features:
- highly improved contrast and sensitivity (< 200 pg)
- very fast and easy 30-minute procedure!
- suitable for 2D and DNA gels
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| Example of a Deep Silver stained SDS-PAGE gel. |
Product description:
Deep Silver combines the sensitivity of conventional silver staining with the speed and ease you ´ve always been waiting for (see your bands 30 minutes after fixing). Whereas old protocols require multiple incubation, washing and development steps, Deep Silver does it all in one single solution! Moreover, contrast and sensitivity have been markedly improved in this kit (down to 200 pg! ). While you got brownish bands on your gels until now, Deep Silver generates a deep black staining color. The silver stain develops over a period of 10 -20 minutes, to give you enough time to stop the reaction at the point of staining intensity you require. The reaction is stopped in EDTA or standard fixing solution and gels can be dried and stored as usual. Deep Silver is suitable for the staining of 2D and DNA gels as well. One kit is sufficient for 40 SDS mini gels.
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Snow White Reversible Zinc Stain
Rapid negative stain for SDS gels
Features:
- crystal clear protein bands
- fast and simple two-step staining procedure
- stained gel can be used for many subsequent procedures such as Western blotting, amino acid analysis, electroelution and MALDI-TOF
Product description:
Snow White is a reversible, rapid negative stain for SDS-PAGE gels. A simple two-step staining procedure allows visualization of bands in just 10 minutes with a sensitivity close to that of silver stain. Snow White produces an opaque white background with crystal clear protein bands that can easily be viewed and documented when placed against a dark background (e.g. a UV screen). The destaining solution of the kit allows complete destaining of the gel in just 15 minutes. Gels can then be applied to subsequent procedures such as Western blotting, electroelution, amino acid analysis, N-terminal sequencing, MALDI-TOF...you name it!
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| Example of a Snow White stained SDS-PAGE gel placed against the dark background of a UV-transilluminator. |
Enlight Fluorographic Enhancer
Allows fluorographic enhancement of low-energy isotopes in polyacrylamide and agarose gels
Product description:
Enlight is a non-toxic aqueous solution that allows the fluorographic enhancement of low-energy isotopes in polyacrylamide and agarose gels. After electrophoresis, the gel is soaked in the Enlight solution for one hour. Then, the gel is dried as usual and exposed to an X-ray film at -70°C. Low-energy electrons that cannot leave the gel matrix are likely to interact with fluorographic molecules which are now incorporated in the gel and stimulate a light-emitting process. These photons can easily leave the gel to expose the film. The increased detection efficiency for 3H is at least 10,000 fold, for 14C, 35S and 33P at least 100-fold! Enlight is stable at room temperature for at least 12 months.
| Comparison of Enlight-treated and -un-treated gels with 14C-labeled protein marker and 3H- and 35S-labeled cell lysates after overnight exposure at -70°C. |  |
NOWA Chemiluminescent Western
Highly sensitive detection system for HRP-conjugated antibodies
Product description:
NOWA (New Optical Western Analysis) is a highly sensitive detection system for HRP-conjugated antibodies in the picogram range. It enables a sensitivity that is significantly higher than that of other leading chemiluminescence kits.
The kit is based on the emission of light during a reaction which is started by free oxygen produced by the enzymatic activity of HRP. Using a newly developed enhancer cascade, the light emission is amplified by a factor of at least one thousand! The NOWA kit contains two solutions that are combined and used for incubating the nitrocellulose or PVDF filter. After one minute of incubation time the filter is ready to be exposed to an X-ray film. Typical exposure times range between 10 seconds and 5 minutes. The light-emitting reaction continues for about 1 hour to allow as many exposures as you may need. The shelf life of the NOWA kit is at least 12 months when stored at 4°C. The kit contains 2 x 250 ml and is sufficient for 10,000 cm2
(1 m2 ) of NC or PVDF membrane.
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| Comparison of light emission of NOWA and popular supplier A´s kit. |
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ProTect - DNA Protector
ProTect is the sun screen for your DNA. Preparative gel electrophoresis of DNA usually includes illumination of the stained gel with UV-B light to identify the bands of interest. However, it is known that even short exposure of DNA to ultraviolet light creates extensive damage to these biomolecules, reducing subsequent reaction efficiencies (ligation, in vitro transcription) dramatically. ProTect circumvents such DNA damage by simply adding it to your gel solution before pouring the gel. ProTect does not reduce or influence the fluorescence properties of the gel - it just protects your DNA!
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see below |
For a detailed data sheet as a pdf-file, please click on the product No.
| ORDER INFORMATION, SHIPPING & STORAGE: | ||
| order# | description | amount |
| DB001 | Deep Blue (50 mini gels) | 0.5 l |
| DB002 | Deep Blue (100 mini gels) | 1 l |
| SS001 | Deep Silver Kit (40 mini gels) | 1 |
| PROT01 | ProTect - DNA Protector | 5 x 2 ml |
| shipped at RT; store at 4°C (Deep Blue can be stored at RT) | ||
| SW001 | Snow White Kit (25 mini gels) | 1 |
| ENL01 | Enlight | 0.5 l |
| ENL02 | Enlight | 1 l |
| NOWA01 | NOWA Kit (2 x 250 ml) | 1 |
| shipped at RT; store at RT (NOWA can be stored at 4°C) | ||
See also chapter 6 of our online catalog.
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