Mobicol "F"

The versatile small column with inserted filter for affinity chromatography!

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	Mobicol "F"
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	MobiSpin Column "F"
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	Mobicol "classic"
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	Accessories
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Features

• Convenient snap off plug
• From a very few to up to 700 µl
  
• Autoclavable at 110 °C for 10 min
  
• Let it drain by gravity flow or spin it
  
• Compatible with minifuges and 1.5 – 2 ml cups
  
• Additional cap with Luer-lock connection

The Mobicol “F” columns for affinity chromatography are compatible to the laboratory standard. With affinity matrix they are convenient for:

• Purification of peptides
  
• Purification of tagged proteins
  
• Purification of native proteins (using immobilized IgG, NTP-binding proteins, β-lactamase, Igase, peroxidase, erythropoietin, β-galactosidase)
  
• Fractionation and purification of antibodies (using protein A/G, immobilized epitopes, peptide matrix)
  
• Purification of glycoproteins, and carbohydrates
• Purification of fatty acids and fatty acid binding proteins
• Whole mount embryo in situ hybridization
  
  

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Related Products

The Mobicol "F" columns are available prefilled with immobilized proteases, nucleases, alk. phosphatase, ß-galactosidase or a compound of your choice on request.

MobiSpin Column "F"

The universal spin column for centrifugation!

Content
	Mobicol "F"
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	MobiSpin Column "F"
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	Mobicol "classic"
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	Accessories
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Features

• Column with filters of 10 µm pore size
  
• Column volume is 700 µl
  
• Fill in the matrix of your choice
  
• Can be centrifuged in 1.5 ml or 2 ml tubes and fits all common centrifuges
  
• Autoclavable at 110 °C for 10 min

The MobiSpin Column "F" is convenient for diverse applications. You can fill in your matrix of your choice and spin the column in every common centrifuge in 1.5 ml oder 2 ml cups. For prefilled MobiSpin Columns "F" please see the related products.
For example you can use MobiSpin Columns "F" with gel filtration matrix for:

• Removal of unicorporated dyes or dye terminators for purification of DNA after labeling reactions
  
• DNA purification prior to sequencing
  
• Removal of free nucleotides
  
• Oligonucleotide purification after synthesis
  

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Related Products

The MobiSpin Columns "F" are available prefilled with: Sephadex^® G50, Sephacryl^® S-200, S-300 and S-400, or a compound of your choice on request.

Mobicol "classic": Centrifugable and Autoclavable 1 ml Columns

The Mobicol "classic" for affinity chromatography and centrifugation!

Content
	Mobicol "F"
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	MobiSpin Column "F"
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	Mobicol "classic"
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	Accessories
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Features

• Can be centrifuged in minifuges
• Autoclavable at 120°C (with filters at 110°C)
• Have Luer-lock connectors
• Are compatible with laboratory standard
• Smallest and largest volumes can be treated easily
• Luer adapters available for tubing
• Can be closed tightly with a screw cap and a plug at the outlet
• Actual volume is 800 µl
  
  

Filters:
Filter of the pore sizes 10 µm, 35 µm or 90 µm are available separately for Mobicol "classic". The filters can be inserted easily with the help of a plastic tool, which is delivered with the columns. The upper large filter fits tightly over the whole length of the column body.

Empty spin columns:
Since Mobicol "classic" can be centrifuged in minifuges, they are also excellent as spin columns. Our empty spin columns come with screw-cap, inserted filter (10 µm) and bottom plug.

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Accessories

Variable Accessories are available for the Mobicols and Lab Columns:

• Tubing-to-Luer-lock adapter for Mobicols or laboratory columns (Fig. A)
• Luer-adapter for the top-cap (M), Luer-adapter for outlet (F) and Luer-lock adapter (M)/(M) (Fig. B)
• One-way stopcocks, Luer-lock (Fig. C)
• Different small and large filters
  
  

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