Vector for PCR* product cloning

products | DNA vector systems | PCR Cloning Vector p3T

DNA Vector Systems

PCR Cloning Vector p3T

Features:

cloning PCR products via multiple dA extensions
results in higher efficiencies than with single dA/dT overhangs

Product Description:

The p3T vector provides a flexible system for the direct cloning of PCR products. It allows cloning of PCR products via multiple dT overhangs.
Utilizing the restriction site Pfl MI, three dT overhangs are produced. After polyadenylating the PCR fragment with Terminal Deoxynucleotidyl Transferase, the PCR fragment can be cloned with high efficiency via a multiple dA extension.

Using p3T, less amplified DNA is required. A Sma I site is present to reduce vector background; Msc I sites flank the insert for optimal excision. Blue/white selection by alpha -complementation is possible. MoBiTec also supplies Terminal Deoxynucleotidyl Transferase including polyadenylation buffer.

*PCR is a process covered by patents owned by Hoffmann-La Roche. Use of this process requires a licence.

Vector Map: p3T vector map

Vector map of p3T; Amp^R, ampicillin resistance; M13 ori, origin of phage M13; lac Z, lac I, from lactose operon; ori, ColE1 origin; MCS, multiple cloning site.

ORDER INFORMATION, SHIPPING STORAGE:
order#	description	amount
P123T	p3T vector DNA, lyophilized	5 µg
shipped at RT; store at 4°C

See also chapter 4 of our online catalog.

Downloads: Handbook, p3T sequence

For further Vector System products please download the Vector Systems brochure (PDF, 3,9 MB)

<< Suicide Vector pCorrectClone

Promoter-Trap Vector pBBR RESO >>