Nucleic Acid Purification

AquaGenomic™

Highest yields and purest total DNA in just 1 hour

Overview

AquaGenomic™ is a novel, nontoxic and multifunctional aqueous solution for the isolation
and purification of genomic DNA. The patent pending technology allows the completion of
cell suspension, cell lysis, DNA extraction and debris removal using a single aqueous solution!

Features:

• It's different
  Isolation and purification is based on selective extraction in aqueous solution instead of binding to solid support.
  
• It's simple
  Cells are lysed, genomic DNA is extracted, and cell debris is precipitated in a single step using one aqueous solution, before purified genomic DNA is precipitated with isopropanol.
  
• It's easy
  No columns, no resins, no slurries, no beads, no filters, no membranes, no needles, no cartridges, no manifolds, no HPLC.
  
• It's scalable
  One single AquaGenomic™ solution does all: mini, midi, maxi and 96-well preps.
  
• It's flexible
  Genomic DNA can be isolated from cultured cells, buccal swabs, microbes, blood, saliva, stool or various animal or plant tissues using the same AquaGenomic™ Solution.
  
• It's fast
  Less than 20 minutes from cells or tissues to pure genomic DNA.
  
• It delivers pure DNA
  Excellent purity - suitable for all common applications.
  
• It delivers high yield
  Excellent quantities of 5-10 µg DNA from 1-2 mio. cells, 200 µl of whole blood, 15 mg of feces, 1 ml bacterial culture, 5-10 mg of animal tissue, or 10-20 mg of plant tissue.
  
• Its economic
  AquaGenomic™ Solution is priced about 1/4 compared with other genomic DNA isolation kits - that’s 85 ct per mini prep!
  

* Depending on kit version

Recommended Applications

• Cultured cells
• Whole blood
• Buccal swabs
• Saliva
• Animal Tissue (liver, lung, kidney, heart, brain, spleen, muscle, tail, etc.)
• Stool
• Plant tissue
• Microbials
• Soil
• Multi-well format
  

• AquaGenomic MSDS
• AquaPrecipi Solution MSDS

• AquaGenomic Handbook (pdf, 1,2 MB, last update 12.2006)
• AquaPrecipi Solution (pdf, 87 KB, last update 09.2009)

AquaRNA™

A novel aqueous solution-based technology for isolation and purification of RNA

AquaRNA™ is a multifunctional aqueous solution for DNA, RNA, and protein extraction. It functions to lyse the cells, inactivate RNases, and extract DNA, RNA, and proteins. DNA and RNA are readily recovered from the lysate by isopropanol precipitation, while proteins remain in the supernatant and can be recovered by acetone precipitation. It allows you to isolate DNA, RNA, and proteins for analyses from the same cells, tissue, or organism. Additionally, AquaRNA™ can be used to inactivate and remove contaminating RNases in purified RNA samples to ensure RNA integrity and stability.

Features:

• Concept-Changing
  AquaRNA™ will change the way you think and work with RNA by simply inactivating and removing endogenous contaminating RNases in your purified RNA samples.
  
• "RNA is very unstable, labile, and must be stored at -80 °C." - This is a misconception. If endogenous contaminating RNases are removed, RNA samples can be stored at 4 °C or even room temperatures for months without degradation.
  
• "We buy and use only RNase-free consumables." - This is a waste of money. If RNA is not contaminated with endogenous RNases, your chance of getting RNA degradation by using regular reagents, solutions, tubes, and tips is very slim.
  
• "RNA work must be done at designated RNA-only workbench and use RNA-only equipment." - This is again a waste of resources. Just don't let your fingers, with or without gloves, touch the inside of your RNA tube and its lid, you will be fine. "I am always anxious until I see the results of my RNA experiments." - If the RNA is not contaminated with residual RNases, you will find that RNA is no longer difficult to work with and you will get consistent and reproducible results.
  
• RNase Decontamination
  AquaRNA™ can be used to treat purified RNA that is contaminated with RNases. It can also be used to treat plasmid DNA or in vitro transcribed RNA to remove any contaminating RNases to ensure successful in vitro transcription and translation reactions.
  
• Not only for RNA
  AquaRNA™ can extract DNA, RNA, and proteins from the same biological samples. If you are comparing two genomic profiles, such as normal vs. tumor tissues, why not compare their RNA expression profiles, and further, their protein expression profiles as well? In addition to maximizing the value of your invaluable samples, you might uncover new correlations and new differences.
  
• Simple
  Due to the multifunctionality of the AquaRNA™ solution, its protocol is very simple. After cell lysis, DNA/RNA are precipitated with isopropanol leaving proteins in the supernatant.
  
• Easy
  No columns, no resins, no slurries, no beads, no filters, no membranes, no needles, no cartridges, no manifolds, no HPLC, no ultracentrifuge.
  
• No Nasty Organic Solvents
  AquaRNA™ extracts DNA/RNA/proteins much like the famous Trizol^® (Invitrogen), but is much simpler and requires no phenol and no chloroform. Yes, it contains guanidine thiocyanate.
  
• Scalable
  One single AquaRNA™ Kit does all: mini, midi, maxi, and HTP; no need to purchase separate mini, midi, maxi, and HTP kits. High yield up to 100 ug of DNA, 50 ug of RNA, and 2000 ug of proteins can be isolated from 5 million mammalian cells or 50 mg animal tissue.
  
• Economic
  AquaRNA™ kit is priced at 199 €/kit for 300 minipreps, that is 0.66 €/miniprep or approximately 1/6 the price of other RNA isolation kits. Each miniprep uses 100 ul AquaRNA to extract DNA/RNA/proteins from 1 million cells.

Facts and figures of AquaRNA™ in comparison with RNA purification kits from a leading competitor. Comparison Main competitor's kits AquaRNA™ kit Isolation Mechanism Selective Binding Selective Extraction Isolation Format Solid Support Aqueous Solution List Price per mini prep 3.89 - 4.44 €* 0.66 - 1.5 € Mercaptoethanol Yes No Scalable No Yes Number of Solutions in the kit 4 1 Hands-on Time (min) 30 30 Yield (µg RNA / 106 cells) 10-20 10-20 RNase contamination ? No Recover Small RNAs No Yes Co-Purify DNA/Proteins Trace Yes Comparison of total RNA isolated from different sources by AquaRNA. Bacterial culture (0.5 ml), human cultured cells (500,000 cells), and rat liver tissue (20 mg) were processed using the AquaRNA Kit. Final DNA/RNA pellet was suspended in 100 ul DEPC water. Five-microliter of each prep treated with or without DNase I was run in a non-denaturing 0.5% native agarose gel, showing the DNA, 28S (23S), 18S (16S), and 5S RNA bands.

Handbook

Download the AquaRNA handbook (pdf, 363 kb)

AquaPreserve™

Preserve and extract DNA, RNA and proteins without crosslinking

AquaPreserve™ is an aqueous solution based preservative with DNA, RNA, and protein extraction functionality. It may be used to streamline biospecimen collection, stabilization, transport, storage, and DNA, RNA, and protein extraction; therefore, it simplifies biospecimen processing, reduces processing heterogeneity, and eliminates artificial pre-analytical changes in a specimen. Both animal and plant tissue specimens and biological fluids, such as whole blood, may be collected in AquaPreserve, shipped and stored at ambient temperatures. DNA and RNA can be then extracted from the preserved biospecimens without using additional extraction kits.

Features

• Preservation and extraction
• Stabilization without crosslinking
• Preservation without freezing
• Extracting DNA, RNA, and proteins without using other extraction kits

There is a significant amount of heterogeneity in the methods used to collect, process, store, and extract biospecimens for molecular analyses. Improper handling of biospecimens may artificially introduce molecular changes in the biospecimens, often without the knowledge of the researchers, which in turn can lead to false research findings or wrong diagnoses. By combining biospecimen preservation and biomolecule extraction with AquaPreserve™, it may be possible to standardize and streamline the entire pre-analytical biospecimen workflow, from biospecimen collection, stabilization, transport, storage, to biomolecule extraction, and thus, prevent or reduce artificially introduced variability in a biospecimen.
Formalin is the most commonly used preservative for stabilizing biospecimens. However, it causes significant damages to the biomolecules by crosslinking DNA, RNA, and proteins in the preserved tissues and could compromise downstream molecular analyses. AquaPreserve™ stabilizes biospecimen by inactivating degradative enzymes without causing biomolecule crosslinking.

Freezing is the gold standard for biospecimen preservation. However, frozen storage requires significant investments not only in space and sophisticated storage and monitoring systems but also in on-going expenses from the high energy and staff costs. Although it is still ongoing to determine how long a biospecimen may be stored in AquaPreserve at ambient temperatures, the inconvenient snap freezing and dry ice shipment can be eliminated by the use of AquaPreserve™.

With AquaPreserve™, you don't need to worry about which kits to use for DNA, RNA, and protein extraction. They can all be accomplished with this single solution. Simply homogenize the specimen in AquaPreserve™ to extract DNA, RNA, and proteins, and then precipitate the DNA/RNA with isopropanol and precipitate the proteins with acetone. It would not only save processing time, labor, and money, but most importantly also prevent pre-analytical variables from using different extraction kits.

AquaStool™

AquaStool™ is a multifunctional aqueous solution for DNA and RNA isolation and purification from fecal biospecimens. This single solution functions to stabilize, preserve, extract fecal DNA/RNA, and remove contaminating PCR and RT-PCR inhibitors abundant in these biospecimens. It streamlines stool collection, stabilization, preservation, DNA/RNA extraction, and molecular analysis. Approximately 150-200 µg total nucleic acids can be isolated from 50 mg of human feces.

Features

• Extract both Fecal DNA and RNA
• Extract DNA and RNA of the Host and Microbes in the Feces
• Not only for Fecal Specimens

Enabling Technology
Stool is a readily accessible, abundant, under-utilized, and noninvasive source of biospecimen. It contains trillions of microorganisms living in our aerodigestive and gastrointestinal tracks, millions of human cells sloughed from these organs every day, and numerous macrophages and lymphocytes migrating between our gut lumen and blood circulation. It can be an unmatched and excellent barometer of our health and diseases, in particular, gastrointestinal cancers, infections, inflammations, and microbiome. However, stool is one of the most difficult and challenging biospecimens to obtain high-quality DNA and RNA for molecular analysis due to its containing of large amount of digestive enzymes that destroy the DNA and RNA prior to and during their extraction, and numerous DNA and RNA contaminants that interfere with subsequent molecular analysis. AquaStool™ technology will facilitate the growth of stool-based sciences and accelerate the drive toward personalized medicine.

Multifunctionalities
AquaStool™ combines the functions of biospecimen stabilization, preservation, DNA/RNA extraction, and PCR inhibitor removal in a single solution. It streamlines biospecimen preservation, DNA/RNA extraction, and molecular analysis. It makes fecal DNA/RNA extraction simple, fast, economic, and convenient.

AquaStool™ extracts both fecal DNA and RNA from the same fecal specimen, enabling noninvasive genotyping and RNAtyping for the identification of transgenic animals; monitoring wildlife animals; diagnosis of gastrointestinal disorders, and the study of RNA expression and its relationship to genetic variation. 

AquaStool™ extracts total nucleic acids in feces, including DNA and RNA from the host, commensal bacteria, any invasive viruses, fungi, or parasites, and incompletely digested foods, enabling forensic identifications; the study of human and animal microbiome; and diagnosis of bacterial, viral, fungal, and parasitic infections.

The challenges we face in DNA and RNA extraction from fecal specimens exceed the challenges we will encounter in most other biospecimens. Therefore, AquaStool™ protocols can be readily modified and adapted to DNA and RNA preservation and extraction from other biomaterials, such as microbes, culture cells, animal and plant tissues, and even insects, making AquaStool™ a universal reagent for the study and correlation of genetic mutation and gene expression of any life form.

MobiSpin Columns for DNA Purification

Features:

• Compatible with laboratory standard
• Column comes pre-packed and equilibrated
• Easy handling: spin, load sample, spin and collect the purified product
• No sample dilution
• Reproducible results with simplified protocols
• One sample in less than 4 minutes
• Numerous samples can be processed simultaneously
• Large number of applications

Product Description:

The MobiSpin columns are designed for a wide variety of nucleic acid purification applications. Next to our new MobiSpin columns with Sephadex^® G-50 resin three different Sephacryl^® resins are available: S-200, S-300 and S-400. See sample volume guide for the selection of the matrix suited best for your specific needs in our online catalog on chapter 6, page 76.

Applications of S-200, S-300 and
S-400 matrices:

• Buffer exchange between enzyme reactions
• DNA purification prior to sequencing
• Oligonucleotide purification after synthesis
• Removal of free nucleotides
• Low cost plasmid purification

Applications of G-50 matrix:

• Removal of unincorporated dyes or dye terminators for purification of DNA after labeling reactions

NuClean Silica Matrix DNA Purification

Features:

• Yields highly purified DNA
  
• Time-saving, easy protocol
  
• Exceptionally low price

Product Description:

NuClean is a quick and efficient system for the purification of single- and double-stranded DNA from solutions and agarose gels. The method is based on the high affinity of nucleic acids to the large surface of the silica particles in the presence of a chaotropic salt. After two washing steps, the purified DNA can be eluted quantitatively in a small volume of water or TE buffer. No further purification is required for subsequent enzymatic procedures. Since DNA fragments <40 bp in size do not bind to the particles, it is possible to separate DNA products from non-incorporated nucleotides and oligonucleotides. Striking features of NuClean are high purity of the nucleic acid product, minimal time needed for the procedure (<20 minutes) and an exceptionally low price! The product yield is between 60 and 80%. One NuClean kit is sufficient for 400 purifications from TAE gels or 300 purifications from reaction solutions. NuClean II comes with an additional modification solution that allows the purification of DNA from TBE gels.

Applications:

• Isolate DNA fragments from TAE or TBE gels
  
• Purify DNA from non-incorporated nucleotides and oligonucleotides
  
• Quickly purify and concentrate your PCR products
  
• Purify plasmids from bacterial lysates

siRNA/dsRNA Purification Kit

The kit is used to remove salts and unincorporated nucleotides from siRNA or dsRNA

Overview

The siRNA/dsRNA Purification Kit is used to remove salts and unincorporated nucleotides from siRNA generated by Dicer enzyme-mediated digestion reactions or dsRNA generated by in vitro transcription reactions.

Features:

• It's ideal for siRNA purification
  Ideal for purifying siRNA generated with the dsRNA-Slicer rec. human Dicer enzyme kit
• It's ideal for dsRNA purification
  Ideal for purifying dsRNA generated with the SpeedScript T7 Transcription Kit
  
• It's easy to handle
  The kit comes with purification columns and hydration buffer.
• It's economic
  Each column is sufficient for purifying 0.5 µg –1.0 µg of siRNA or dsRNA generated with the above mentioned kits.
  
  

dsRNA Removal siRNA Purification Column

The Purification Column is used to remove undigested dsRNA from siRNA.

Overview

dsRNA Removal siRNA Purification Column is used to remove undigested dsRNA from siRNA generated by Dicer enzyme-mediated digestion reactions. It is ideal for purifying d-siRNA generated with the dsRNA-Slicer rec. human Dicer enzyme kit.

Features:

• It's ideal for d-siRNA purification
  It is ideal for purifying d-siRNA generated with the dsRNA-Slicer rec. human Dicer enzyme kit.
  
• It's economic
  Each column is sufficient for purifying 0.5 µg - 1.0 µg of siRNA generated with the above mentioned kit.

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