HS-Nuclease, recombinant Endonuclease
HS-Nuclease is a recombinant form of Serratia marcescens extracellular endonuclease (encoded by the same gene as Bezonase®) produced in E. coli using a proprietary process. This nonspecific endonuclease hydrolyzes both single- and double-stranded nucleic acids (DNA and RNA) to 5’-phosphorylated oligonucleotides of 1 - 4 bases in length. HS-Nuclease is a highly purified homodimer of 27 kDa subunits that has exceptional high specific activity and is free of protease activity. HS-Nuclease is ideal to digest nucleic acids and to reduce viscosity during protein purification and sample preparation.
Recombinant RNase T1
Ribonuclease T1 (RNase T1) is an endoribonuclease that specifically cuts RNA at the 3'-end of guanosine residues through a 2', 3'-cyclic phosphate intermediate mechanism. RNase T1 is cloned and produced recombinantly to create a highly pure enzyme without contaminating DNase or nonspecific RNase activity.
RNase T1 is used for RNA structure and mapping studies and for RNA protection assays, as well as for removal of RNA from DNA samples.
Recombinant RNase T2
Ribonuclease T2 (RNase T2) is an endoribonuclease that c atalyzes the two-stage endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides terminating in guanylic 3'-phosphate with 2',3'-cyclic phosphate intermediates.
Recombinant Exonuclease III
Exonuclease III (ExoIII) of E. coli is a 31 kD monomeric, globular protein combining multiple catalytic activities in one active site. It acts on doublestranded (ds) DNA as a 3'-5' exonuclease, a 3'- phosphomonoesterase, an apurinic/apyrimidimic (AP) sites specific endonuclease and an exonucleolytic ribonuclease H. Of particular interest for molecular biological methods is its exonucleolytic activity, removing 5'-mononucleotides from the 3'-hydroxyl ends of ds DNA, leaving protruding 5'-termini. Its catalytic rate can be adjusted by temperature and NaCl concentration and thus allows for the generation of single-stranded DNA templates for sequencing or recombination methods.
Applications
- Construction of nested unidirectional deletions of DNA fragments in combination with nuclease S1
- Generation of a single-stranded template for dideoxy sequencing of DNA
- Site-directed mutagenesis
- Cloning of PCR products
- In vitro recombination
Recombinant Exonuclease III-T
Engineered exonuclease III with improved temperature resistance.
Applications
- Construction of nested unidirectional deletions of DNA fragments in combination with nuclease S1
- Generation of a single-stranded template for dideoxy sequencing of DNA
- Site-directed mutagenesis
- Cloning of PCR products
- In vitro recombination
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