For reverse transcription and mRNA transcription!

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RNA Transcription

AMV Reverse Transcriptase

M-MuLV Reverse Transcriptase

AMV Reverse Transcriptase

For reverse transcription of smaller RNA templates and dideoxy sequencing

Product Description

AMV Reverse Transcriptase is isolated from the Avian Myeloblastosis Virus as the alpha beta holoenzyme of 157 kDa. AMV Reverse Transcriptase has both, a 5' 3' primer-dependent polymerase activity which uses either RNA or DNA as a template, and a 3' 5' RNase H activity that degrades the RNA from RNA/DNA hybrids. It requires a primer as well as Mg²⁺ or Mn²⁺ ions. MoBiTec's AMV Reverse Transcriptase can be applied to dideoxy sequencing of DNA and RNA and to cDNA synthesis using smaller RNA templates. In cDNA reactions, the enzyme is compatible with 4 mM sodium pyrophosphate and placental RNase inhibitor. The optimal temperature is 41-45°C. A temperature of 37-41°C is preferable if NaPPi is used, however. MoBiTec's AMV Reverse Transcriptase is delivered together with a 10 X reaction buffer.

Applications

reverse transcription (RT-PCR) and cDNA synthesis
RNA sequencing and dideoxy sequencing of DNA
primer extension and 3'-end labeling of DNA fragments

order information

M-MuLV Reverse Transcriptase

High fidelity reverse transcription of long RNA templates - RNase

Product Description

Moloney Murine Leukemia Virus (M-MuLV) Reverse Transkriptase is isolated from an E. coli strain that carries a plasmid with the cloned modified M-MuLV RT gene with deleted RNAse H coding part. M-MuLV RTase is an RNA-dependent DNA polymerase. This enzyme can synthesize a complementary DNA strand initiating from a primer using either single-stranded RNA or DNA template. The enzyme lacks RNAse H activity.

Applications

reverse transcription and first strand cDNA synthesis
primer extension

For a detailed data sheet as a pdf-file, please click on the product No.

ORDER INFORMATION, SHIPPING & STORAGE:
order#	description	amount
02040G	M-MuLV Reverse Transkriptase, RNase H (100 U/ µl)	1,000 U
02041G	M-MuLV Reverse Transkriptase, RNase H (100 U/ µl)	5,000 U
02042G	M-MuLV Reverse Transkriptase, RNase H (100 U/ µl)	10,000 U
02014G	AMV Reverse Transkriptase (20U/µl)	250 U
02015G	AMV Reverse Transkriptase (20U/µl)	500 U
02016G	AMV Reverse Transkriptase (20U/µl)	4 x 250 U
shipped on dry ice; store at -20°C

See also chapter 6 of our online catalog.

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