products | nucleic acid and protein tools | Endoproteinase
Pro-Pro-Y-Pro Igase
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Nucleic Acid and Protein Tools
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Endoproteinase Pro-Pro-Y-Pro (Igase) |
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Endoproteinase Lys/Arg-Arg (Kex) |
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Endoproteinase Ile-Glu-Gly-Arg (Factor Xa Protease) |
Endoproteinase Pro-Pro-Y-Pro Igase
Features:
- highly efficient specific endoproteinase
- cleaves amino acid sequence N-X-Z-Pro-Pro/-Y-Pro-C
(X = preferably Pro or Ser;Y = Thr,Ser or Ala; Z = preferably Arg or Thr) - also cleaves insoluble aggregates derived from inclusion bodies
- used for cleavage of fusion proteins (see Fusion Protein System PheBo)
- affinity of endoproteinase Pro-Pro-Y-Pro to Phenylboronate columns facilitates separation of enzyme and substrate after digestion
- natural substrate is IgA1
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| Cleavage site of endoproteinase Pro-Pro-Y-Pro; schematic drawing. |
Product Description:
In recombinant protein technology sequence-specific enzymatic cleavage of fusion proteins has become an important application. Endoproteinase Pro-Pro-Y-Pro, a protein of 106 kDa, efficiently processes polypeptides at authentic or engineered sites. Since the endoproteinase cleaves the proline-rich hinge region of IgA1 the enzyme is also referred to as Igase or IgA protease 4. Endoproteinase Pro-Pro-Y-Pro recognizes the amino acid sequence. The highly specific proteolysis can be obtained not only with soluble and purified protein fusions but also with insoluble aggregates derived from cytoplasmic inclusion bodies.
| ORDER INFORMATION, SHIPPING & STORAGE: | ||
| order# | description | amount |
| EP0205 | Endoproteinase Pro-Pro-Y-Pro (approx. 250 µg/ml) |
50 µg |
| shipped on dry ice; store at -20°C | ||
See also chapter 6 of our online catalog.
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